ELECTROPHYSIOLOGY June 12 – July 1
Section Description: The objective of the electrophysiology section is to learn electrophysiology from first principles to practical application. Students will receive lectures from leading scientists that range from the basics of whole cell recordings to ion channel structure/function to the molecular basis and modulation of synaptic transmission. Students will rotate through a series of experimental preparations that include the invertebrate neuromuscular junction, single channel recording and analysis in cultured cells, whole cell currents in cultured cells, and in vivo patch clamp recordings in tissue slices and intact central nervous system and glutamate uncaging. Rotations will be followed by independent research projects. These projects will be short, original research projects designed and executed under the guidance of individual teams of faculty and TAs. The goal is attain a basic understanding of the theory and application of a wide range of modern electrophysiological techniques and the application of these techniques toward solving outstanding questions in modern neuroscience.
Electrophysiology Readings (Dropbox Link) – use course login
Course Lectures (Dropbox link)
Tutorial Simulations and Other Educational Materials
Neuroscience Online – A surprisingly good free online textbook.
Neurons in Action – A great set of simulations of basic electrophysiological principles. Not free, but we will install it in some of the lab computers.
Nernst/Goldman Equation Simulator – Web-based simulator of N/G equation, useful for understnading concept of resting membrane potential.
Simulations of ion channels and excitable membranes – A useful online simulation from Pancho Benzanilla to better understand channel function. Uses outdated Java, so may not work.
MetaNeuron– A free computer program desinged to simulate passive and active membrane properties and synaptic transmission. Very intuitive.
Neuron– A fully-featured neuron simulator used in several research labs. Links to program, documentation and datasets can be found here. Best of all, it’s FREE!
SimePhys – Not very well developed ephys experiment simulator
SNNAP – Simulator for neural networks and action potentials
Electrophysiology of the Neuron – My favorite one, if you can get it to run on your machine.
Neuron Simulation Lab – Various ell designed tutorial simulating various single cell experiments, free from Amrita University, in India.
NeuroElectro – Database of various electrophysiological properties from various cell types.
NeuroLex – Morphology Database of various cell types, useful for modelling.
NeuroMorpho.org – Another morphology database
Allen Brain Atlas (Cell Types) – Database of electrophysiology and morphology properties from lots of cells. Not many cells yet, but very usable data and interface.
Daily Schedule (some exceptions noted below)
9:00 – 12:00: Lecture
12:00 – 1:30 pm: Lunch/ Free time
1:30 – 6:00 pm: Lab
6:00 – 7:00 pm: Dinner/Free time
7:00 pm – evening: Lab and/or evening seminar
June 12 – Sun 6pm-9pm: Bar-B-Q in front of Loeb. Meet Physiology Faculty
June 13 – Mon 9am-12:00 Lecture: Jon Sack – Introduction to Voltage Clamp
1:30-6pm: Model Cell Demo, Rig tour
8:00pm Monday Night Lecture: Kathy Nagel, NYU
June 14 – Tues 9am-12:00 Lecture: Jon Sack – Single Channel Recording
June 15 – Wed 9am-12:00 Lecture: Ellen Lumpkin – Mechanotransduction
June 16 – Thurs 9am-12:00 Lecture: Bruce Bean – Voltage Gated Channels
June 17 – Fri 9am-12:00 Lecture: Rod McKinnon – Structure of Ion Channels
June 18 – Sat OFF
June 19 – Sun OFF
June 20 – Mon 9am-12:00 Lecture: Diana Bautista- TRP Channels, Itch
8:00pm Monday Night Lecture: Diana Bautista – UC Berkeley
June 21 – Tues 9am-12:00 Lecture: Carlos Aizenman – Modulation of intrinsic excitability
Evening: Projects Meeting and Beer!
June 22 – Wed 9am-12:00 Lecture: Dan Johnston – Dendritic Integration
Evening: Ephys Review – Breakout Session
June 23 – Thur 9am-12:00 Lecture: Chris Moore – Optogenetics and Neural Dynamics
June 24 – Fri 9am-12:00 Lecture: Ricardo Araneda – Olfactory Processing
June 25 – Sat Day/Evening: Projects
June 26 – Sun OFF
June 27 – Mon 9am- 12:00 Lecture: Mauro Costa-Mattioli – Synaptic Plasticity
8:00pm Monday Night Lecture: Mauro Costa-Mattioli, Baylor College of Medicine
June 28 – Tues 9am-12:00 Lecture: Martin Muller – Ca2+ Signaling & Synaptic Transmission
June 29 – Wed 9am-12:00 Lecture: Patrick Kanold – Development of Neural Circuits
4:30-6pm: Meeting with Electron Microscopy Faculty
June 30 – Thur 9am-12:00: Grae Davis – Homeostatic Signaling
1:30-evening: Last day of recordings, end at dinner. Presentation prep, data analysis, etc.
July 1 – Fri 9am-12:00: No Lecture, cleanup.
3:00-5:30 pm: Presentations (followed by photo)
6:30pm: End of Cycle Party
July 2 – Sat OFF
July 3 – Sun OFF (start of Imaging Cycle Bar-B-Q in evening, 6pm Loeb Patio)
Carlos Aizenman Brown University Carlos_Aizenman@brown.edu
Kara Pratt University of Wyoming email@example.com
Ellen Lumpkin Columbia Univ. firstname.lastname@example.org
Diana Bautista UC Berkeley email@example.com
Ricardo Araneda Univ. Maryland firstname.lastname@example.org
Jon Sack UC Davis email@example.com
Martin Mueller Univ. Zurich firstname.lastname@example.org
Patrick Kanold Univ. of Maryland email@example.com
Mark Lillya Team Sack; firstname.lastname@example.org
Ruilong Hu Team Araneda; email@example.com
Theanne Griffith Team Lumpkin; firstname.lastname@example.org
Blair Jenkins Team Lumpkin; email@example.com
Zhenyu Liu Team Pratt/Aizenman; firstname.lastname@example.org
Sebastien Sydik Team Mueller; email@example.com
Meg Younger Team Mueller; Meg Younger <firstname.lastname@example.org>
Rongkang Deng Team Kanold; email@example.com
Bruce Bean Harvard Medical School
Rod MacKinnon Rockefeller University
Diana Bautista Univ. California Berkeley
Chris Moore Brown University
Dan Johnston UT Austin
Mauro Costa-Mattioli Baylor College of Medicine
Kathy Nagal New York University